Bus Gainesville, FL to Knoxville, TN

Compare all timetables for the bus from Gainesville, FL to Knoxville, TN

Here is a selection of the cheapest bus trips from the schedule from Gainesville, FL to Knoxville, TN from various bus operators like FlixBus or Greyhound US for the next days.

today

tomorrow

Sunday

$149

Monday

Tuesday

$150

Wednesday

$141

Thursday

Cheapest bus trips Sunday
BertCompanyCountOneCompanyName	Duration	Departure time	From	Departure	Arrival time	To	Destination	Seat classes	Price
FlixBus	21h 30m	1:10 AM	Gainesville, FL	Rosa Parks Transfer station	10:40 PM	Knoxville, TN	Greyhound Bus Station	Economy	$149	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/21/2025 1:10:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/21/2025 10:40:00 PM. 50 Economy ticket for $149 per person are available. Travel duration is 21h 30m
Greyhound US	13h 50m	8:00 AM	Gainesville, FL	Greyhound Bus Station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$149	A Bus from Greyhound US goes from Greyhound Bus Station, Gainesville, FL (USA) at 12/21/2025 8:00:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/21/2025 9:50:00 PM. 50 Economy ticket for $149 per person are available. Travel duration is 13h 50m
FlixBus	23h 10m	10:40 PM	Gainesville, FL	Rosa Parks Transfer station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$156	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/21/2025 10:40:00 PM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/22/2025 9:50:00 PM. 50 Economy ticket for $156 per person are available. Travel duration is 23h 10m

Cheapest bus trips Tuesday
BertCompanyCountOneCompanyName	Duration	Departure time	From	Departure	Arrival time	To	Destination	Seat classes	Price
FlixBus	21h 30m	1:10 AM	Gainesville, FL	Rosa Parks Transfer station	10:40 PM	Knoxville, TN	Greyhound Bus Station	Economy	$150	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/23/2025 1:10:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/23/2025 10:40:00 PM. 50 Economy ticket for $150 per person are available. Travel duration is 21h 30m
FlixBus	23h 10m	10:40 PM	Gainesville, FL	Rosa Parks Transfer station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$154	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/23/2025 10:40:00 PM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/24/2025 9:50:00 PM. 50 Economy ticket for $154 per person are available. Travel duration is 23h 10m
Greyhound US	13h 50m	8:00 AM	Gainesville, FL	Greyhound Bus Station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$158	A Bus from Greyhound US goes from Greyhound Bus Station, Gainesville, FL (USA) at 12/23/2025 8:00:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/23/2025 9:50:00 PM. 50 Economy ticket for $158 per person are available. Travel duration is 13h 50m

Cheapest bus trips Wednesday
BertCompanyCountOneCompanyName	Duration	Departure time	From	Departure	Arrival time	To	Destination	Seat classes	Price
FlixBus	23h 10m	10:40 PM	Gainesville, FL	Rosa Parks Transfer station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$141	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/24/2025 10:40:00 PM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/25/2025 9:50:00 PM. 50 Economy ticket for $141 per person are available. Travel duration is 23h 10m
FlixBus	21h 30m	1:10 AM	Gainesville, FL	Rosa Parks Transfer station	10:40 PM	Knoxville, TN	Greyhound Bus Station	Economy	$151	A Bus from FlixBus goes from Rosa Parks Transfer station, Gainesville, FL (USA) at 12/24/2025 1:10:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/24/2025 10:40:00 PM. 50 Economy ticket for $151 per person are available. Travel duration is 21h 30m
Greyhound US	13h 50m	8:00 AM	Gainesville, FL	Greyhound Bus Station	9:50 PM	Knoxville, TN	Greyhound Bus Station	Economy	$153	A Bus from Greyhound US goes from Greyhound Bus Station, Gainesville, FL (USA) at 12/24/2025 8:00:00 AM to Greyhound Bus Station, Knoxville, TN (USA) arriving at 12/24/2025 9:50:00 PM. 50 Economy ticket for $153 per person are available. Travel duration is 13h 50m

Best Price Offer: The price for the cheapest bus from Gainesville, FL to Knoxville, TN is $122 on 12/18/2025. Flexible travelers save on ticket prices.

Frequency of bus connections between Gainesville, FL and Knoxville, TN

See here how frequent the connections between Gainesville, FL and Knoxville, TN are, during different times and days of the week.

Sunday is the busiest day, with more than 3 daily buses from Gainesville, FL to Knoxville, TN. Sunday has instead the fewest connections, with only 3 daily coaches between Gainesville, FL and Knoxville, TN.

The busiest time of the day is the Morning, with 3 buses from Gainesville, FL to Knoxville, TN, while Morning sees the fewest connections between Gainesville, FL and Knoxville, TN, with 3 offers.

Facts about the bus from Gainesville, FL to Knoxville, TN

Compare all providers like FlixBus and Greyhound US that travel 3 times every day by bus from Gainesville, FL to Knoxville, TN in one click! Book your bus ticket from Gainesville, FL to Knoxville, TN starting from $122!

Cheapest Bus	$122
Fastest Bus	13h 50m
Earliest Bus	1:10 AM
Latest Bus	10:40 PM
Daily Bus Routes	3 Ø
Distance	446.4 miles
Bus Companies	FlixBus and Greyhound US

Cheapest bus connections from Gainesville, FL to Knoxville, TN

Every day, 3 buses from 2 bus companies leave Gainesville, FL for Knoxville, TN: in the table below, you will find the cheapest prices for a bus ticket for this route, starting from 12/19/2025 and for the following days.

Sunday

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday 12/14

Monday 12/15

Tuesday 12/16

Wednesday 12/17

Thursday 12/18

Friday 12/19

Saturday 12/20

Sunday 12/21

$149

Monday 12/22

Tuesday 12/23

$150

Wednesday 12/24

$141

Thursday 12/25

Friday 12/26

Saturday 12/27

Sunday 12/28

$175

Monday 12/29

Tuesday 12/30

Wednesday 12/31

Thursday 01/01

Friday 01/02

Saturday 01/03

Sunday 01/04

Monday 01/05

Tuesday 01/06

Wednesday 01/07

Thursday 01/08

Friday 01/09

Saturday 01/10

The cheapest time to travel from Gainesville, FL to Knoxville, TN

See here on which day and which time of the day you can find the cheapest bus ticket from Gainesville, FL to Knoxville, TN, for the next 30 days.

With a price starting from $141, Wednesday is the cheapest day for a bus from Gainesville, FL to Knoxville, TN. Tuesday instead is the most expensive day with prices starting from $150.

Evening is the cheapest day time for bus tickets from Gainesville, FL to Knoxville, TN, with $141. Morning is the most expensive time of the day with prices starting from $149.

How to save money travelling from Gainesville, FL to Knoxville, TN

Travel can be expensive, but it doesn't need to be! Do you want to avoid paying a terrible price for your trip? Then follow these quick steps to save money:

Book the ticket from Gainesville, FL to Knoxville, TN in advance! The earlier you book, the cheaper usually the price is. Also, you will be sure to have a place on the bus from Gainesville, FL to Knoxville, TN, compared instead if you buy it at the last moment, or directly at the station.

If you can, avoid travelling at peak times. Instead of weekend, try travelling during the week. Travelling in the evening or at night it’s also cheaper, and later buses from Gainesville, FL to Knoxville, TN are also emptier.

All bus stations and stops in Gainesville, FL and Knoxville, TN

In the map below, you can see where to find all bus stations in Gainesville, FL and Knoxville, TN.

Rosa Parks Transfer station

SE 3rd St 700

North Walmart Supercenter Bus Stop

Westgate Plaza Bus Stop

Central Bus Station

University of Florida (Commuter Lot)

Gale Lemerand Dr

Kingston Pike

Kingston Pike 6927

Knoxville Area Transit Bus Stop

Knoxville Area Transit Bus Stop Knoxville Area Transit Bus Stop

Walker Springs Plaza Shopping Center

Kingston Pike 8509

Greyhound Bus Station

E Magnolia Ave 100

Transit Center

E Church Ave 301

Service and Comfort on the bus from Gainesville, FL to Knoxville, TN

Want to know which seat classes are available on the buses from Gainesville, FL to Knoxville, TN? Or which bus companies serve the route Gainesville, FL to Knoxville, TN? Below you will find answers to your questions.

FAQs about the Gainesville, FL to Knoxville, TN bus

How much does a Gainesville, FL Knoxville, TN bus trip cost?

This depends on, among other things, the day and time of travel. The cheapest trip is available from $122 and takes 23h 10m to reach Knoxville, TN. Compare offers to save money!

How much could I save by comparing buses from Gainesville, FL to Knoxville, TN?

This route is offered by 2 companies with a range of ticket prices between $122 and $158. Thus, we recommend booking your ticket as early as possible to make sure you get the cheapest bus ticket to Knoxville, TN.

On average, how many connections are available from Gainesville, FL to Knoxville, TN each day?

Every day, the Gainesville, FL to Knoxville, TN bus route is served by approximately 3 connections. With our search, you can compare bus times to find the perfect trip.

How long does a bus journey from Gainesville, FL to Knoxville, TN take?

The fastest bus from Gainesville, FL to Knoxville, TN is offered by Greyhound US and takes 13h 50m. Bus tickets for this connection cost $149. In this context, bear in mind that the journey can take longer due to traffic.

What time is the first bus from Gainesville, FL to Knoxville, TN?

The earliest bus arriving in Knoxville, TN from Gainesville, FL starts at 1:10 AM. However, information regarding departure times may differ on weekends and holidays. Simply use our search to find exact times for your planned travel date.

What time is the last bus from Gainesville, FL to Knoxville, TN?

The latest bus trip departing from Gainesville, FL for Knoxville, TN starts at 10:40 PM. Please note that departure times may differ on weekends and holidays. So just use our search to find the exact times for your planned travel date.

Which bus companies operate the Gainesville, FL Knoxville, TN route?

When traveling by bus from Gainesville, FL to Knoxville, TN, passengers can travel with FlixBus and Greyhound US. With our search, you can compare all available bus tickets to book the one that works for you.

Is there a direct bus between Gainesville, FL and Knoxville, TN?

You can check all the direct connections between Gainesville, FL and Knoxville, TN by using our search and looking at the Changes section in our filters. These are some of the companies which offer a direct bus: FlixBus and Greyhound US.

What can I take with me on the Gainesville, FL to Knoxville, TN bus route?

Depending on which provider you travel with, what you're allowed to bring with you can vary. Generally, you can take a suitcase and hand luggage with you on the Gainesville, FL Knoxville, TN bus route free of charge. Simply use our search to find a bus that is best suited to your needs for your planned travel date.

What equipment is available for the Gainesville, FL Knoxville, TN bus route?

The equipment on board differs depending on the provider. However, many long-distance buses have WiFi so you can tell your friends your exact location during the journey. To make traveling even more pleasant, air conditioning systems provide ventilation. It is best to use our search to get precise information about the equipment of the bus from Gainesville, FL to Knoxville, TN on your planned travel date.

How does CheckMyBus find the best bus deals from Gainesville, FL to Knoxville, TN?

CheckMyBus collaborates directly with companies around the world to provide you with the best options when searching for a trip. We retrieve their first-hand data to show you real-time prices, schedules, and seat availability for 3 daily trips between Gainesville, FL and Knoxville, TN. With the most up-to-date information from verified sources, you can fairly compare all available options and choose the trip that suits you best.

How does CheckMyBus technology work for trips from Gainesville, FL to Knoxville, TN?

Prices and availability for 3 trips between Gainesville, FL and Knoxville, TN change constantly. To provide you with the most up-to-date information, we connect directly with bus operators, Online Travel Agencies (OTAs), and Global Distribution Systems (GDS) via real-time interfaces (APIs). The data collected from these sources undergoes strict quality checks, is processed within our infrastructure, and is then presented to you on our search results pages. Visit our About Us page to learn more.

Available seat classes which are offered on the bus route Gainesville, FL to Knoxville, TN

Economy

The cheapest option available for your ticket, it usually comes with reclinable seats and AC.

More bus routes to Gainesville, FL and to Knoxville, TN

Here’s an overview of the most popular bus routes to Gainesville, FL and to Knoxville, TN. Simply click on the connection of your choice, and you will find all the details about your bus route!